fluidigm custom primers Search Results


custom sequencing primers  (fluidigm)
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fluidigm custom sequencing primers
Custom Sequencing Primers, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96 96 dynamic gene expression ifc  (fluidigm)
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96 96 Dynamic Gene Expression Ifc, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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custom designed deltagene assay primer pairs  (fluidigm)
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fluidigm custom designed deltagene assay primer pairs
Single HV + Cells Are Totipotent in the Ground State, Related to <xref ref-type=Figure 4 Single cell qRT-PCR was carried out on a BioMark HD System (Fluidigm) using a 96 × 96 chip. DELTAgene Assay custom designed primers (Fluidigm) were used for amplification. Technical replicates were generated for each single cell. (A) Histograms showing the distribution of expression of 4 genes in each population. Data shown unnormalized. (B) Single cells were sorted by flow cytometry into LIF withdrawal conditions. After 7 days differentiated colonies were immunostained for GATA6, CDX2 and BRACHYURY. Quantification of marker expression patterns. 2 and 3 marker exclusive expression indicates colonies that contain independent cells positive for different lineage markers. (C) Whole-mount immunostaining of an E6.5 chimeric embryo generated by injection of a single HV+ ES cell constitutively expressed H2B-Tomato, previously cultured in 2i/LIF, into a morula stage wild-type embryo. Upper whole-mount image shows an extended focus view of the entire embryo. Panels below show optical sections, generated by confocal microscopy, with ES cells contributing to trophoblast and visceral and parietal endoderm (VE and PE, respectively). (D) E6.5 chimeras generated by injection of a single HV- cell that had been cultured in 2i/LIF. The majority of single cells contributed only to epiblast (11/13), with only 2 showing extraembryonic contribution. " width="250" height="auto" />
Custom Designed Deltagene Assay Primer Pairs, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/custom designed deltagene assay primer pairs/product/fluidigm
Average 93 stars, based on 1 article reviews
custom designed deltagene assay primer pairs - by Bioz Stars, 2026-06
93/100 stars
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96 96 dynamic array ifc  (fluidigm)
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fluidigm 96 96 dynamic array ifc
Single HV + Cells Are Totipotent in the Ground State, Related to <xref ref-type=Figure 4 Single cell qRT-PCR was carried out on a BioMark HD System (Fluidigm) using a 96 × 96 chip. DELTAgene Assay custom designed primers (Fluidigm) were used for amplification. Technical replicates were generated for each single cell. (A) Histograms showing the distribution of expression of 4 genes in each population. Data shown unnormalized. (B) Single cells were sorted by flow cytometry into LIF withdrawal conditions. After 7 days differentiated colonies were immunostained for GATA6, CDX2 and BRACHYURY. Quantification of marker expression patterns. 2 and 3 marker exclusive expression indicates colonies that contain independent cells positive for different lineage markers. (C) Whole-mount immunostaining of an E6.5 chimeric embryo generated by injection of a single HV+ ES cell constitutively expressed H2B-Tomato, previously cultured in 2i/LIF, into a morula stage wild-type embryo. Upper whole-mount image shows an extended focus view of the entire embryo. Panels below show optical sections, generated by confocal microscopy, with ES cells contributing to trophoblast and visceral and parietal endoderm (VE and PE, respectively). (D) E6.5 chimeras generated by injection of a single HV- cell that had been cultured in 2i/LIF. The majority of single cells contributed only to epiblast (11/13), with only 2 showing extraembryonic contribution. " width="250" height="auto" />
96 96 Dynamic Array Ifc, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/96 96 dynamic array ifc/product/fluidigm
Average 95 stars, based on 1 article reviews
96 96 dynamic array ifc - by Bioz Stars, 2026-06
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deltagene assay custom designed primers  (fluidigm)
93
fluidigm deltagene assay custom designed primers
Single HV + Cells Are Totipotent in the Ground State, Related to <xref ref-type=Figure 4 Single cell qRT-PCR was carried out on a BioMark HD System (Fluidigm) using a 96 × 96 chip. DELTAgene Assay custom designed primers (Fluidigm) were used for amplification. Technical replicates were generated for each single cell. (A) Histograms showing the distribution of expression of 4 genes in each population. Data shown unnormalized. (B) Single cells were sorted by flow cytometry into LIF withdrawal conditions. After 7 days differentiated colonies were immunostained for GATA6, CDX2 and BRACHYURY. Quantification of marker expression patterns. 2 and 3 marker exclusive expression indicates colonies that contain independent cells positive for different lineage markers. (C) Whole-mount immunostaining of an E6.5 chimeric embryo generated by injection of a single HV+ ES cell constitutively expressed H2B-Tomato, previously cultured in 2i/LIF, into a morula stage wild-type embryo. Upper whole-mount image shows an extended focus view of the entire embryo. Panels below show optical sections, generated by confocal microscopy, with ES cells contributing to trophoblast and visceral and parietal endoderm (VE and PE, respectively). (D) E6.5 chimeras generated by injection of a single HV- cell that had been cultured in 2i/LIF. The majority of single cells contributed only to epiblast (11/13), with only 2 showing extraembryonic contribution. " width="250" height="auto" />
Deltagene Assay Custom Designed Primers, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/deltagene assay custom designed primers/product/fluidigm
Average 93 stars, based on 1 article reviews
deltagene assay custom designed primers - by Bioz Stars, 2026-06
93/100 stars
  Buy from Supplier
fluidigm custom primers  (fluidigm)
93
fluidigm fluidigm custom primers
Single HV + Cells Are Totipotent in the Ground State, Related to <xref ref-type=Figure 4 Single cell qRT-PCR was carried out on a BioMark HD System (Fluidigm) using a 96 × 96 chip. DELTAgene Assay custom designed primers (Fluidigm) were used for amplification. Technical replicates were generated for each single cell. (A) Histograms showing the distribution of expression of 4 genes in each population. Data shown unnormalized. (B) Single cells were sorted by flow cytometry into LIF withdrawal conditions. After 7 days differentiated colonies were immunostained for GATA6, CDX2 and BRACHYURY. Quantification of marker expression patterns. 2 and 3 marker exclusive expression indicates colonies that contain independent cells positive for different lineage markers. (C) Whole-mount immunostaining of an E6.5 chimeric embryo generated by injection of a single HV+ ES cell constitutively expressed H2B-Tomato, previously cultured in 2i/LIF, into a morula stage wild-type embryo. Upper whole-mount image shows an extended focus view of the entire embryo. Panels below show optical sections, generated by confocal microscopy, with ES cells contributing to trophoblast and visceral and parietal endoderm (VE and PE, respectively). (D) E6.5 chimeras generated by injection of a single HV- cell that had been cultured in 2i/LIF. The majority of single cells contributed only to epiblast (11/13), with only 2 showing extraembryonic contribution. " width="250" height="auto" />
Fluidigm Custom Primers, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluidigm custom primers/product/fluidigm
Average 93 stars, based on 1 article reviews
fluidigm custom primers - by Bioz Stars, 2026-06
93/100 stars
  Buy from Supplier

Image Search Results


Single HV + Cells Are Totipotent in the Ground State, Related to <xref ref-type=Figure 4 Single cell qRT-PCR was carried out on a BioMark HD System (Fluidigm) using a 96 × 96 chip. DELTAgene Assay custom designed primers (Fluidigm) were used for amplification. Technical replicates were generated for each single cell. (A) Histograms showing the distribution of expression of 4 genes in each population. Data shown unnormalized. (B) Single cells were sorted by flow cytometry into LIF withdrawal conditions. After 7 days differentiated colonies were immunostained for GATA6, CDX2 and BRACHYURY. Quantification of marker expression patterns. 2 and 3 marker exclusive expression indicates colonies that contain independent cells positive for different lineage markers. (C) Whole-mount immunostaining of an E6.5 chimeric embryo generated by injection of a single HV+ ES cell constitutively expressed H2B-Tomato, previously cultured in 2i/LIF, into a morula stage wild-type embryo. Upper whole-mount image shows an extended focus view of the entire embryo. Panels below show optical sections, generated by confocal microscopy, with ES cells contributing to trophoblast and visceral and parietal endoderm (VE and PE, respectively). (D) E6.5 chimeras generated by injection of a single HV- cell that had been cultured in 2i/LIF. The majority of single cells contributed only to epiblast (11/13), with only 2 showing extraembryonic contribution. " width="100%" height="100%">

Journal: Cell Reports

Article Title: Totipotent Embryonic Stem Cells Arise in Ground-State Culture Conditions

doi: 10.1016/j.celrep.2013.04.034

Figure Lengend Snippet: Single HV + Cells Are Totipotent in the Ground State, Related to Figure 4 Single cell qRT-PCR was carried out on a BioMark HD System (Fluidigm) using a 96 × 96 chip. DELTAgene Assay custom designed primers (Fluidigm) were used for amplification. Technical replicates were generated for each single cell. (A) Histograms showing the distribution of expression of 4 genes in each population. Data shown unnormalized. (B) Single cells were sorted by flow cytometry into LIF withdrawal conditions. After 7 days differentiated colonies were immunostained for GATA6, CDX2 and BRACHYURY. Quantification of marker expression patterns. 2 and 3 marker exclusive expression indicates colonies that contain independent cells positive for different lineage markers. (C) Whole-mount immunostaining of an E6.5 chimeric embryo generated by injection of a single HV+ ES cell constitutively expressed H2B-Tomato, previously cultured in 2i/LIF, into a morula stage wild-type embryo. Upper whole-mount image shows an extended focus view of the entire embryo. Panels below show optical sections, generated by confocal microscopy, with ES cells contributing to trophoblast and visceral and parietal endoderm (VE and PE, respectively). (D) E6.5 chimeras generated by injection of a single HV- cell that had been cultured in 2i/LIF. The majority of single cells contributed only to epiblast (11/13), with only 2 showing extraembryonic contribution.

Article Snippet: For this experiment, a BioMark HD System (Fluidigm) was used with custom designed DELTAgene Assay primer pairs (Fluidigm; sequences are provided in ).

Techniques: Quantitative RT-PCR, Amplification, Generated, Expressing, Flow Cytometry, Marker, Immunostaining, Injection, Cell Culture, Confocal Microscopy